Run (50 µg/mL in 50% methanol):
The 20AV enables simultaneous deuterium and tungsten lamp operation for high-sensitivity tracking across the entire ultraviolet-visible range.
By learning the operational logic of your SPD-20A through your manual and pairing it with a proactive maintenance strategy, you ensure your detector consistently delivers the high-quality data your work demands.
If the cell is heavily contaminated, pass 0.1 M Nitric Acid ( HNO3cap H cap N cap O sub 3 shimadzu spd20a user manual better
Optimizing Your HPLC Analysis: Getting More Than Just the Basics from the Shimadzu SPD-20A User Manual
Principles for a Better Manual
[System Power ON] ➡️ [Verify Flow < 0.5 mL/min] ➡️ [Ignite D2 Lamp] ➡️ [30-Min Equilibrium] ➡️ [Execute Autozero] Run (50 µg/mL in 50% methanol): The 20AV
By moving beyond the standard operating procedures, you ensure: Better detection limits for trace analysis. Improved Accuracy: Reproducible peak shapes and areas.
Technical reference (appendices)
Before diving into the documentation, it's useful to recap what the SPD-20A is capable of. Part of the Prominence series, the SPD-20A is a UV-Vis detector renowned for its high sensitivity and stability. It features a noise level of just (0.5 \times 10^-5) AU and an industry-leading wide linearity of 2.5 AU. This performance makes it suitable for detecting trace impurities, such as a 0.05% concentration relative to the main compound in a sample. Improved Accuracy: Reproducible peak shapes and areas
| Setting | Default | Better Value | When | |---------|---------|--------------|------| | Time constant | 1.0 sec | 0.5 sec | Fast peaks (UHPLC) | | AUFS | 0.1 | 0.05 | Trace analysis | | Cell temp | Off | 35°C | Improve reproducibility | | Wavelength slit | 8 nm | 4 nm | Higher resolution (less noise) |
: The SPD-20A can monitor two different wavelengths simultaneously, which is critical for peak purity checks and quantifying complex mixtures. Temperature Control